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The Journals of Gerontology Series A: Biological Sciences and Medical Sciences Advance Access published online on April 14, 2009
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, doi:10.1093/gerona/glp045
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© The Author 2009. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org.

Methodology for Discovery of Alzheimer’s Disease Blood-Based Biomarkers

Olivier C. Maes1, Hyman M. Schipper1,2,3, Howard M. Chertkow1,2,3 and Eugenia Wang4,5

1 Centre for Neurotranslational Research, Bloomfield Centre for Research in Aging, Lady Davis Institute for Medical Research, Montréal, Québec, Canada
2 Department of Neurology and Neurosurgery
3 Department of Medicine (Geriatrics), McGill University, Montréal, Québec, Canada
4 Gheens Center on Aging
5 Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Kentucky

Address correspondence to Eugenia Wang, PhD, Gheens Center on Aging, University of Louisville, 580 South Preston Street, Louisville, KY 40202. Email: eugenia.wang{at}louisville.edu


  Abstract

Alzheimer's disease (AD) is a degenerative brain disorder. The disease also affects peripheral tissue such as peripheral blood mononuclear cells (PBMCs). Delineating biochemical alterations in AD blood constituents may possibly allow the identification of accessible footprints that reflect degenerative processes within the central nervous system. Here, we describe an integrated methodology for the generation of a blood-based molecular bio-repository, including the collection of clinical and demographic data for downstream stringent sample selection and stratification for the study of molecular signatures in AD. We report the simultaneous extraction of high quality and yield of DNA, RNA, and protein from PBMCs of individuals with sporadic AD, mild cognitive impairment, and normal elderly controls. We describe experimental designs and present examples for the discovery of underlying etiopathogenetic networks in sporadic AD. We suggest that PBMC-associated biomarkers may provide insights into the pathogenesis of AD and be used to monitor disease diagnosis and progression.

Keywords Bio-repository; Biomarker; Peripheral blood mononuclear cell; Sporadic Alzheimer’s disease; Mild cognitive impairment

Received: August 5, 2008; Accepted: February 25, 2009

Decision Editor: Huber R. Warner, PhD


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