Skip Navigation



The Journals of Gerontology Series A: Biological Sciences and Medical Sciences Advance Access published online on February 4, 2009
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, doi:10.1093/gerona/gln056
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
64A/4/419    most recent
gln056v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Bloomer, S. A.
Right arrow Articles by Kregel, K. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bloomer, S. A.
Right arrow Articles by Kregel, K. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org.

Differential Regulation of Hepatic Heme Oxygenase-1 Protein With Aging and Heat Stress

Steven A. Bloomer1, Hannah J. Zhang1, Kyle E. Brown2,3,4 and Kevin C. Kregel1,4

1 Department of Integrative Physiology, University of Iowa, Iowa City
2 Iowa City Veterans Administration Medical Center, Iowa City
3 Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City
4 Program in Free Radical and Radiation Biology, Department of Radiation Oncology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City

Address correspondence to Kevin C. Kregel, PhD, Department of Integrative Physiology, 532 FH, University of Iowa, Iowa City, IA 52242. Email: kevin-kregel{at}uiowa.edu


  Abstract

Increased expression of heme oxygenase-1 (HO-1) in response to physiological stress is considered to be a protective response, which may be altered with aging. In this study, HO-1 expression was assessed following heat stress by immunoblotting of liver homogenates and isolated hepatocytes from young (6 months) and old (24 months) Fischer 344 rats and by immunohistochemistry. Livers of old rats showed higher baseline levels of HO-1, which was predominately localized to Kupffer cells. After heat stress, young animals showed a greater relative increase in hepatic HO-1, part of which was caused by increased numbers of nonparenchymal cells that were immunoreactive to HO-1. Consistent with these data, HO-1 was significantly upregulated after hyperthermia in vitro only in hepatocytes from young rats. Hence, aging alters stress-induced expression of HO-1 in a cell-specific manner, which may contribute to the diminished stress tolerance observed in older organisms.

Keywords Oxidative stress; Kupffer cells; Inflammation; Hyperthermia

Received: August 14, 2008; Accepted: November 13, 2008

Decision Editor: Huber R. Warner, PhD


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.