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The Journals of Gerontology Series A: Biological Sciences and Medical Sciences Advance Access originally published online on May 22, 2009
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences 2009 64A(9):927-939; doi:10.1093/gerona/glp066
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© The Author 2009. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org.

Impact of Lifelong Sedentary Behavior on Mitochondrial Function of Mice Skeletal Muscle

Pedro A. Figueiredo1, Scott K. Powers2, Rita M. Ferreira1, Francisco Amado3, Hans J. Appell1,4 and José A. Duarte1

1 Centro de Investigação em Actividade Física, Saúde e Lazer, Faculty of Sports, University of Porto, Portugal
2 Department of Applied Physiology and Kinesiology, University of Florida, Gainesville
3 Department of Chemistry, University of Aveiro, Aveiro, Portugal
4 Department of Physiology and Anatomy, German Sport University, Cologne, Germany

Address correspondence to Pedro A. Figueiredo, MSc, Centro de Investigação em Actividade Física, Saúde e Lazer, Faculty of Sports, University of Porto, Rua Dr. Plácido Costa, 91, 4200-450 Porto, Portugal. Email: pfigueiredo{at}ismai.pt


  Abstract

This study investigated the impact of lifelong sedentariness on skeletal muscle mass and mitochondrial function. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young-Y; old sedentary-OS; old active-OA). Young animals were sacrificed after 1 week of quarantine, and OS and OA groups were individually placed into standard cages and in cages with running wheels, respectively, until sacrifice (25 months). Body weights and hind-limb skeletal muscle wet weights were obtained from all groups. Mitochondrial respiratory functional measures (i.e., state 3 and 4 respiration, respiratory control ratio, and ratio of nanomoles of ADP phosphorylated by nanomoles of O2 consumed [ADP/O]) and biochemical markers of oxidative damage (aconitase activity, protein carbonyl derivatives, sulfhydryl groups) were measured in isolated mitochondrial suspensions. Our results reveal that lifelong sedentary behavior has a negative impact on the age-related loss of skeletal muscle mass and on the isolated mitochondrial function of mixed skeletal muscle of mice, which is associated with an increased oxidative damage to mitochondrial biomolecules.

Keywords Voluntary activity; Muscle aging; Sarcopenia; Running wheel; Oxidative stress

Received: November 25, 2008; Accepted: April 21, 2009

Decision Editor: Huber R. Warner, PhD


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