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The Journals of Gerontology Series A: Biological Sciences and Medical Sciences Advance Access originally published online on May 15, 2009
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences 2009 64A(8):839-849; doi:10.1093/gerona/glp064
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© The Author 2009. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org.

A Proteomics Analysis of the Effects of Chronic Hemiparetic Stroke on Troponin T Expression in Human Vastus Lateralis

Jeffrey P. Rabek1, Charlene E. Hafer-Macko2, James K. Amaning1, James H. DeFord1, Vincent L. Dimayuga1, Mark A. Madsen3, Richard F. Macko2,4 and John Papaconstantinou1

1 Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston
2 Department of Neurology, University of Maryland School of Medicine, Baltimore
3 The Scripps Institute for Research, La Jolla, California
4 Department of Medicine, Geriatrics Division, University of Maryland School of Medicine, Baltimore

Address correspondence to John Papaconstantinou, PhD, Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX 77555-0643. Email: jpapacon{at}utmb.edu


  Abstract

Stroke disability is attributed to upper motor neuron deficits resulting from ischemic brain injury. We have developed proteome maps of the Vastus lateralis to examine the effects of ischemic brain injury on paretic skeletal muscle myofilament proteins. Proteomics analyses from seven hemiparetic stroke patients have detected a decrease of three troponin T isoforms in the paretic muscle suggesting that myosin–actin interactions may be attenuated. We propose that ischemic brain injury may prevent troponin T participation in complex formation thereby affecting the protein interactions associated with excitation–contraction coupling. We have also detected a novel skeletal troponin T isoform that has a C-terminal variation. Our data suggest that the decreased slow troponin T isoform pools in the paretic limb may contribute to the gait deficit after stroke. The complexity of the neurological deficit on Vastus lateralis is suggested by the multiple changes in proteins detected by our proteomics mapping.

Keywords Troponin T isoforms; Skeletal muscle proteome; 2D gel electrophoresis; Stroke

Received: April 19, 2008; Accepted: March 30, 2009

Decision Editor: Huber R. Warner, PhD


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