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The Journals of Gerontology Series A: Biological Sciences and Medical Sciences Advance Access originally published online on February 5, 2009
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences 2009 64A(1):21-33; doi:10.1093/gerona/gln048
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© The Author 2009. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org.

Aging Impairs Skeletal Muscle Mitochondrial Bioenergetic Function

Pedro A. Figueiredo1, Scott K. Powers2, Rita M. Ferreira1, Hans Joachim Appell1,3 and José A. Duarte1

1 CIAFEL, Faculty of Sports, University of Porto, Portugal
2 Department of Applied Physiology and Kinesiology, University of Florida, Gainesville
3 Department of Physiology and Anatomy, German Sport University, Cologne, Germany

Address correspondence to Pedro Alexandre Figueiredo, MSc, CIAFEL, Faculty of Sports, University of Porto, Rua Dr. Plácido Costa, 91, 4200-450 Porto, Portugal. Email: pfigueiredo{at}ismai.pt


  Abstract

This study investigated the influence of age on the functional status of mitochondria isolated from skeletal muscle of C57BL/6 mice aged 3 and 18 months. We hypothesized that skeletal muscle mitochondria isolated from aged animals will exhibit a decreased respiratory function. Mitochondrial respiratory functional measures (ie, State 3 and 4 respiration, respiratory control ratio and number of nanomoles of ADP phosphorylated by nanomoles of O2 consumed per mitochondrion) and biochemical markers of oxidative damage (aconitase activity, protein carbonyl derivatives, sulfhydryl groups, and malondialdehyde) were measured in isolated mitochondrial suspensions. Along with traditional tests of mitochondrial function, an in vitro repetitive ADP-stimulation test was used to evaluate the mitochondrial capacity to reestablish the homeostatic balance between successive ADP stimulations. The number of mitochondria per mitochondrial suspension, calculated by transmission electron microscopy, was used to normalize functional and biochemical data. Our results confirm the existence of an age-associated decline in mitochondrial function of mixed skeletal muscle, which is significantly correlated with higher levels of mitochondrial oxidative damage.

Keywords Aging; Mitochondria; Respiratory function

Received: March 6, 2008; Accepted: August 27, 2008

Decision Editor: Huber R. Warner, PhD


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