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The Journals of Gerontology Series A: Biological Sciences and Medical Sciences Advance Access published online on February 4, 2009
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, doi:10.1093/gerona/gln053
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© 2009 The Authors.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Advancing Age Alters the Contribution of Calcium Release From Smooth Endoplasmic Reticulum Stores in Superior Cervical Ganglion Cells

Erik J. Behringer1, Conwin K. Vanterpool2, William J. Pearce1, Sean M. Wilson1 and John N. Buchholz1

1 Department of Physiology and Pharmacology, Loma Linda University, School of Medicine, California
2 Department of Biological Sciences, Alabama A&M, Normal

Address correspondence to John N. Buchholz, PhD, Department of Physiology and Pharmacology, Loma Linda University, School of Medicine, 11041 Campus St., Loma Linda, CA 92354. Email: jbuchholz{at}llu.edu


  Abstract

In superior cervical ganglion (SCG) neurons calcium-induced calcium release (CICR), mediated by ryanodine receptors (RyRs), contributes to stimulation-evoked intracellular calcium ([Ca2+]i) transients. Hypothesis: The contribution of CICR to electrical field stimulation (EFS)–evoked [Ca2+]i transients in SCG cells declines with senescence and may be partially recovered in the presence of caffeine. We measured EFS-evoked [Ca2+]i transients in isolated fura-2–loaded SCG cells from Fischer-344 rats aged 6, 12, and 24 months with either the RyR antagonist ryanodine to block the contribution of CICR to [Ca2+]i transients or caffeine to sensitize CICR to EFS. EFS-evoked [Ca2+]i transients increased from 6 to 12 months and declined at 24 months and ryanodine decreased [Ca2+]i transients in SCG cells from 6- and 12-month-old animals only. Caffeine significantly increased EFS-evoked [Ca2+]i transients in all age groups. These data suggest that CICR declines with senescence and residual CICR function may be reclaimed in senescent cells with caffeine.

Keywords Ryanodine receptors; Aging and calcium release; Aging and function of superior cervical ganglia

Received: August 13, 2008; Accepted: October 22, 2008

Decision Editor: Huber R. Warner, PhD


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